活性氧自由基在ARDS发病机制中作用的实验研究

Experimental study of reactive oxygen species in the pathogenesis of ARDS

目的 探讨活性氧 (ROS)自由基在急性呼吸窘迫综合征 (ARDS)发病机制中的作用。方法 采用电子自旋共振 (ESR)技术直接测量ROS ,并结合超微弱发光法、化学发光法等技术分析鉴定了ROS的种类。结果 ESR结果显示 ,大鼠注射OA后 10minROS含量即升高 ,30min达到高峰 ,以后逐渐下降 ;利用超微弱发光法 ,投用不同ROS的清除剂得出OA型ARDS发病中起主要作用的ROS是超氧阴离子 (O·-2 ) ,而化学发光法定量检测结果与ESR波谱显示的结果完全一致。结论 ROS的改变早于肺毛细血管内皮 (PCEC)损伤和病理形态的发生 ,ROS可能是ARDS启动的主要病因 ;OA型ARDS发病中起主要作用的ROS是O·-2 。

Objective To investigate the role of reactive oxygen species(ROS)in the pathogenesis of acute respiratory distress syndrome(ARDS).Method ROS was measured by electron spin resonance(ESR)directly and the species of ROS were identified and quantified by the ultra-weak luminescence and chemical luminescence.Result The spectrum of ESR showed that the concentration of ROS increased at10minutes and got summit at30minutes after injection of oleic acid(OA),then dropped gradually;the scaveng-ing effect of different scavenging agent to ROS in the experiment of ultra-weak luminescence proved that superoxide anion play an impor-tant role in the development of ARDS induced by chemicals,moreover,the results of quantified measure to superoxide anion by chemi-cal luminescence corresponded with the tendency exhibited in ESR measurement.Conclusion The results demonstrated that the change of ROS was earlier than that of pulmonary capillary endothelial cells(PCEC)and pathophysiological change,ROS may be a key factor in the starting phase of ARDS and superoxide anion may be a leading actor in the action of ROS.It would be helpful to use superoxide dismutase(SOD)and the analog of SOD in the future clinical treatment of ARDS because SOD is the special scavenging agent to super-oxide anion.