自制抗人雄激素受体单克隆抗体检测肝癌标本中AR的表达

PREPARATION OF ANTIHUMAN ANDROGEN RECEPTORMONOCLONAL ANTIBODIES AND DETECTINGTHE EXPRESSION OF AR IN HUMANHEPATOCELLULAR CANCER

目的 :探讨原发性肝癌病人雄激素受体表达 ,进一步评价自行研制抗人雄激素受体 ( androgenreceptor,AR)单克隆抗体 ( Mc Ab)。方法 :采用免疫组化法检测 3 5例原发性肝癌病人雄激素受体。结果 :原发性肝癌病人雄激素受体 71 %为阳性 ,癌组织与癌周围组织相比 ,AR表达有显著差异 ( P<0 .0 1 )。自制 AR Mc Ab与进口 AR多抗存在正相关 r=0 .87,P <0 .0 0 1。结论 :原发性肝癌病人雄激素受体表达与肿瘤大小及分化程度存在相关性 ,可为原发性肝癌激素治疗提供初步资料 ;自制抗 AR Mc Ab可代替进口 AR多抗应用于临床及科研工作。

Objective: To explore the expression of androgen receptor (AR) in human hepatocellular cancer(HCC) and evaluate the preparation of antihuman monoclonal antibodies(McAb). Methods: The status of 35 samples of human (HCC) were studied by immunohistochemistry(IHC) using the anti-hAR McAb. Results: The positive rate of AR expression in HCC tissues was 71%. The positivity were closely correlated with Zymed hAR polyclonal antibodies(r=0 87, P<0 001). Conclusion: The results show that the exprssion of AR in human HCC is a correlated with tumor size and differentiation and the McAb obtain in this study would be a useful tool to detect the AR status in HCC.