肝癌DC疫苗活化的CTL对人肝癌裸鼠皮下移植瘤的抑制作用

Cytotoxic lymphocytes primed by DC based hepatocellular carcinoma vaccine against growth of carcinoma xenograft on nude mice

目的:本研究探讨肝癌DC疫苗活化的CTL对人肝癌裸鼠皮下抑制瘤的抑制作用。方法:将负载肝癌相关抗原DC经BCG HSP70活化后制成肝癌DC疫苗,用此肝癌DC疫苗诱导的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤,同时观察DC对人肝癌裸鼠皮下移植瘤的直接预防作用和冷冻复苏后肝癌特异性淋巴细胞对人肝癌裸鼠皮下移植瘤的预防和治疗作用。结果:DC对肝癌细胞的抑制率为97％,用冷冻复苏后的CTL预防人肝癌裸鼠皮下移植瘤,对肝癌细胞的平均抑制率为96％;冷冻复苏后的CTL治疗人肝癌裸鼠皮下移植瘤,对肝癌细胞的抑制率为98％。结论;本研究结果为肝癌DC疫苗的进一步深入研究及今后的临床应用奠定良好的基础。

AIM:TO investigate the cytotoxic lymphocyte (CTL) primed by DC based hepatocellular carcinoma (HCC) vaccine against the growth of the carcinoma xenograft on nude mice. METHODS:High purity of dendritic cells (DCs) were obtained and the immune responses of lymphocytes primed by BCG HSP70 activated DCs were loaded with HCC associated antigens to prepare DC based HCC vaccine. The nude mouse model bearing human HCC xenografts was established and the characteristics of the tumor were identified with immunohistochemical techniques. Transplanted human HCC on nude mouse was treated with freezing and anabiotic HCC specific lymphocytes. RESULTS:Lymphocytes primed by DCs based HC vaccine had the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotics could prevent and treat HCC xenograft on nude mice with a preventive rate of 97％. CONCLUSION:DCs not only inhibit the growth of human HCC and other cancer cells in vitro, but also prevent the growth of HCC xenograft on nude mice in vivo.