COX-2基因表达与胃癌淋巴结转移的关系

Correlation of COX-2 expression to lymph node metastasis in human gastric carcinoma

目的 研究COX-2 mRNA及蛋白在胃癌组织中的表达,探讨COX-2与胃癌淋巴结转移的关系。方法 采用 RT-PCR方法检测30例新鲜胃癌标本及相匹配的癌旁正常组织COX-2 mRNA的表达;用免疫组化方法检测45例胃癌石蜡标本COX-2蛋白表达。结果 胃癌组织中存在COX-2 mRNA(73.3％)及蛋白(62.2％)表达。伴淋巴结转移者,COX-2 mRNA及蛋白表达高于无转移者,(P<0.05)。结论 COX-2基因在胃癌中表达增高,且与胃癌淋巴结转移明显相关。

Objective To test the expression of cyclooxygenase-2(COX-2) mRNA and protein in gastric carcinoma and to investigate the involvement of COX-2 expression in the lymph node metastasis of human gastric carcinoma. Methods Reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemical methods were used to examine the expression of COX-2 mRNA and protein in gastric carcinoma. Results Expression of COX-2 mRNA and protein were detected in gastric carcinoma (73 .3% ,62.2%) .Expression of COX-2 in gastric carcinoma with lymph node metastasis was significantly higher than that without metastasis ( P < 0. 05). Conclusions COX-2 expression in gastric carcinoma was associated with lymph node metastasis.