PCNA、P^(53)与胎儿食管上皮细胞增殖特性的关系

Expression of PCNA and P^(53) in fetal esophageal epithelium and their relationship with cell proliferation

目的 初步探讨增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)和抑癌基因p53与胎儿食管上皮细胞增殖特性的关系。方法 采用免疫组化ABC法检测胎儿食管组织中PCNA和p53的表达状况。结果PCNA在31例胎儿标本中全部呈阳性反应,PCNA单位面积平均阳性细胞数为506±239/mm^2,与成人不同类型食管上皮组织单位面积PCNA免疫阳性细胞数相比,差异具有显著性,P<0.05。p53免疫组化染色阳性细胞核呈棕黄色,胞浆未见阳性反应,其阳性率为10％(3/31)。结论 PCNA和p53在胎儿食管上皮组织中的表达可反映胎儿食管上皮旺盛的增殖特性。

Objective To study the expression of PCNA and p53 in fetal esophageal epithelium and their relationship with cell proliferation . Methods Immunohistochemical avdin-biotin peroxidase complex (ABC) technique was used to detect the expression of PCNA and tumor suppressor gene p53 in fetal esophagus. Results PCNA immunostain-positive cells were observed in all fetal esophageal samples. The average number of positive immunoreactivity cells per mm is 506±239. There were significant differences( P < 0.05) between the fetal PCNA positive immunoreacivity cells with different stage of adult esophageal epithelia. Positive immunoreactivity cells of r were located in the nuclei in dark brown, and the positive rate was 10% (3/31). Conclusions The expression of PCNA and P53 reflect the active proliferative status of fetal esophageal epithelium.