碳青霉烯类抗生素对产酶革兰阴性杆菌药敏分析

Drug sensitivity of carbapenem antibiotics to enzyme-producing gram negative bacilli

目的 了解 2 5 0株G-杆菌产ESBLs和AmpC酶的状况 ,比较产酶菌与非产酶菌对碳青霉烯类和其他11种抗生素的耐药率 ,为临床治疗产酶菌引起的感染提供理论依据。方法 应用纸片扩散确证法和头孢西丁三维试验分别检测ESBLs和AmpC酶。用K B琼脂扩散法做药敏试验。结果 2 5 0株G-杆菌中 ,5 3株 (2 1 2 % )产AmpC酶 ,98株 (39 2 % )产ESBLs ,6株 (2 4 % )同时产AmpC +ESBLs。碳青霉烯类抗生素对ESBLs菌株 ,AmpC菌株和AmpC +ESBLs菌株的耐药率分别为 3 1% ,3 8%和 0 ,较其它抗生素低。结论 对产酶菌引起的感染应首选碳青霉烯类抗生素。

Objective: To investigate AmpC enzyme and extended spectrum β lactamases production in 250 strains of gram negative bacilli and compare the resistant rates of enzyme producing strains and non enzyme producing strains to carbapenem antibiotic and other 11 antibiotics in order to provide the theoretical evidence for treating infection caused by enzyme producing strains Methods: Disk diffusion confirmation and Cefoxitin three dimensional test were used to detect ESBLs and AmpC enzyme respectively Kirby Bauer agar diffusion was used to judge drug sensitivity Results: Fifty three(21 2%) strains of AmpC and 98(39 2%) for ESBLs, 6(2 4%) strains for ESBLs+AmpC were found in 250 strains of gram negative bacilli The resistant rates of carbapenem antibiotic to strains of AmpC, ESBLs and AmpC+ESBLs were 3 1%, 3 8% and 0 respectively, which were much lower than those of others Conclusion: Carbapenem antibiotics are the most effective antibiotics to the infections caused by enzyme producing strains