HPLC法测定乙烷磺酰胺的含量

Determination of ethane sulphonamide by HPLC

目的 应用高效液相色谱法对乙烷磺酰胺进行含量测定。方法 选用ZORBOXSIL填充的不锈钢柱(2 5 0mm× 4 6mm ,i d ,5 μm) ,水— 2 5 %氨水 (99 5∶0 5 )为流动相 ,流速 1 0ml·min-1,室温 ,检测波长 2 10nm。结果 乙烷磺酰胺在 0 6～ 1 4mg·ml-1范围内线性关系良好 ,r =0 9999(n =5 ) ,平均回收率为 99 83% ,RSD为0 32 %。结论 该方法便捷、准确 。

Objective:To determine ethane sulphonamide by HPLC method Methods:The liquid chromatography was carried out by use of ZORBAX SIL column (250 mm×4 6 mm,i d,5 μm) with detection wavelength at 210 nm The flow rate of mobile phase (water-25% ammonia water,99 5∶0 05) was 1 0 ml·min -1 Results: There was a good linear relationship within the range of 0 6～1 4 mg·ml -1 (r=0 9999,n=5) The average recovery was 99 83% and RSD was 0 32% Conclusion: The method is convenient,accurate and reproducible