期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

成牙本质细胞中上游刺激因子1表达的原位杂交研究 被引量：2

In situ Hybridization Analysis of USF1mRNA Expression in Odontoblasts
下载PDF
导出
摘要 目的检测、证实成牙本质细胞中上游刺激因子1(USF1)mRNA的表达。方法用前期制备的T—USF1质粒作模板,借助特异性引物PCR扩增USF1cDNA片段,回收、随机引物法地高辛标记;培养成牙本质细胞MDPC-23,制备细胞爬片,原位杂交方法检测。结果成牙本质细胞胞浆中有较强的蓝紫色颗粒。结论首次证实体外培养的成牙本质细胞中有USF1mRNA表达,为深入研究USF1分子对成牙本质细胞生长、分化和矿化影响提供了实验依据。 Objective To detect and verify USF1mRNA expression in odontoblasts.Methods T-USF1clone prepared previously was used as template and the desired USF1cDNA segment was amplified by PCR with specific primers.The segment was labeled with digoxin as a probe and in situ hybridization was performed on the mounting of odontoblasts.Results Evidently positive staining was shown in the cytoplasm of odontoblasts.Conclusion We verified the expression of USF1mRNA in odontoblasts for the first time and provided evidence for further research.
作者 吴礼安 文玲英 杨富生 刘勇
机构地区 第四军医大学口腔医学院儿童口腔科
出处 《上海口腔医学》 CAS CSCD 2003年第2期120-122,共3页 Shanghai Journal of Stomatology
关键词 成牙本质细胞 上游刺激因子1 原位杂交 MRNA PCR 细胞生长 体外培养 Odontoblasts USF1 mRNA In-situ hybridization PCR
分类号 R780.2 [医药卫生—口腔医学]
  • 相关文献

参考文献8

  • 1吴礼安,文玲英,杨富生,樊淑梅.牙胚中上游刺激因子部分序列的cDNA克隆[J].牙体牙髓牙周病学杂志,2002,12(11):588-590. 被引量：4
  • 2Sawadogo M,Roeder RG.Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region [J]. Cell, 1985, 43(1): 165-175.
  • 3Henrion A A, Vaulont S, Raymondjean M, et al. Mouse USF1 gene cloning: comparative organization within the c-myc gene family[J].Mammalian Genome, 1996, 7(11): 803-809.
  • 4Ebara S, Kawasaki S, Nakamura I, et al. Transcriptional regulation of the mBMP-4 gene through an E-box in the 5' -flanking promoter region involving USF [J]. Biochem Biophys Res Commun,1997, 240(1): 136-141.
  • 5Sirito M, Lin Q,Deng JM, et al. Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice [J]. Proc Natl Acad Sci, 1998, 95(7): 3758-3763.
  • 6Narayanan K, Srinivas R, Ramachandran A, et al. Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1 [J]. Proc Natl Acad Sci,2001, 98(8): 4516-4521.
  • 7Sun ZL, Fang DN, Wu XY, et al. Expression of dentin sialoprotein(DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23 [J]. Connect Tissue Res, 1998, 37(3-4): 251-261.
  • 8Bronckers AL, Engelse MA, Cavender A, et al. Cell-specific patterns of Cbfal mRNA and protein expression in postnatal murine dental tissues[J]. Mech Dev, 2001, 101(1-2): 255-258.

二级参考文献11

  • 1Viollet B, Lefrancois-Martinez AM, Henrion A, et al. Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms J. J Biol Chem,1996,271(3): 1405
  • 2Qyang Y, Luo X, Lu T, et al. Cell-type-dependent activity of the ubiquitous transcription factor USF in cellular proliferation and transcriptional activationJ. Mol Cell Biol,1999,19(2): 1508
  • 3Sirito M, Lin Q, Deng JM, et al. Overlapping roles and asymmetrical cross-regulation of the USF proteins in miceJ. Proc Natl Acad Sci USA,1998, 95(7):3758
  • 4Sawadogo M, Roeder RG. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionJ. Cell, 1985, 43(1): 165
  • 5Gregor PD, Sawadogo M, Roeder RG. The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimmerJ. Genes Dev,1990, 4(10): 1730
  • 6Luo X, Sawadogo M. Antiproliferative properties of the USF family of helix-loop-helix transcription factorsJ. Proc Natl Acad Sci USA,1996,93(3): 1308
  • 7Ebara S, Kawasaki S, Nakamura I, et al. Transcriptional regulation of the mBMP-4 gene through an E-box in the 5'-flanking promoter region involving USFJ. Biochem Biophys Res Commun, 1997, 240(1): 136
  • 8Nagavarapu U, Danthi S, Boud RT. Characterization of a rat neuronal nicotinic acetylcholine receptor alpha7 promoterJ. J Biol Chem,2001,276(20): 16749
  • 9Zhao Z, Stock D, Buchanan A, et al. Expression of Dlx genes during the development of murine dentitionJ. Dev Genes Evol, 2000,210(5): 270
  • 10Jen Y, Manova K, Benezra R. Expression patterns of Id1, Id2, and Id3 are highly related but distinct from that of Id4 during mouse embryogenesisJ. Dev Dyn,1996, 207(3): 235

共引文献3

同被引文献27

  • 1Sawadogo M,Van Dyke MW,Gregor PD,et al.Multiple forms of the human gene-specific transcription factor USF.I.Complete purification and identification of USF from HeLa cell nuclei.J Biol Chem,1988,263(24):11985-11993.
  • 2Sirito M,Lin Q,Maity T,et al.Ubiquitous expression of the 43-and 44-kDa forms of transcription factor USF in mammalian cells.Nucleic Acids Res,1994,22(3):427-33.
  • 3Laurila PP,Naukkarinen J,Kristiansson K.Genetic association and interaction analysis of USF1 and APOA5 on lipid levels and atherosclerosis arteriosclerosis.Thrombosis and Vascular Biology,2010,30:346-352.
  • 4Pajukanta P,Lilja HE.Familial combined hyperlipidemia is associated with upstream transcription factor 1 (USF1).Nature Genetics,2004,36:371-376.
  • 5Lee JC,Weissglas-Volkov D.USF1 contributes to high serum lipid levels in Dutch FCHL families and U.S.whites with coronary artery disease Arteriosclerosis.Thrombosis and Vascular Biology,2007,27:2222-2227.
  • 6Nowak M,Helleboid-Chapman A,Jake H,et al.Insulin-mediated down-regulation of apolipoprotein A5 gene expression through the phosphatidylinositol 3-kinase pathway:role of upstream stimulatory factor.Mol Cell Biol,2005,25(4):1537-1548.
  • 7Sirito M,Lin Q,Jian MD,et al.Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice.Proc Natl Acad Sci USA,1998,95(7):3758-3763.
  • 8Zhu J,Giannola DM,Zhang Y,NF-Y cooperates with USF1/2 to induce the hematopoietic expression of HOXB4.Blood,2003,102(7):2420-2427.
  • 9Giannola DM,Shlomchik WD,Jegathesan M,et al.Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2.The Journal of experimental medicine,2002,192(10):1479-1490.
  • 10Liang SY,Moghimi B,Crusselle-Davis VJ,et al.Defective erythropoiesis in transgenic mice expressing dominant-negative upstream stimulatory factor.Mol Cell Biol,2009,29(21):5900-5910.

引证文献2

二级引证文献1

上海口腔医学
2003年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部