舌鳞癌细胞系Tca8113 Fas表达水平与凋亡的关系

Relationship of Apoptosis and Expression of Fas Gene in Tongue Squamous Cell Carcinoma Cell Line Tca8113

目的研究舌鳞癌Tca8113细胞Fas表达水平与细胞凋亡的关系,了解细胞凋亡的相关机制。方法采用脂质体法将含Fas基因片段的真核表达重组质粒pBK-Fas导入舌鳞癌Tca8113细胞,检测转染前后FasmRNA表达水平、Fas蛋白阳性表达率和阳性表达强度。以及细胞凋亡指数,分析细胞凋亡的相关因素。结果Fas转染不提高舌鳞癌Tca8113细胞Fas蛋白阳性表达率(P>0.05),但能提高FasmRNA表达水平、Fas蛋白表达强度,以及细胞凋亡指数。各项指标分别由转染前的0.201±0.015,31.02±4.63,0.88%±0.16%上升到0.399±0.073,54.32±6.38和10.21%±1.46%,统计分析均有显著性差异(P<0.01)。结论Fas介导Tca8113凋亡与Fas蛋白阳性率可能无关,而与Fas蛋白阳性强度有关。Fas蛋白激活细胞凋亡可能存在阈值。只有Fas表达达到一定强度,细胞凋亡才被激活。

Objective To investigate the relationship of apoptosis and expression of Fas gene in oral aquamous cell carcinoma.Methods Plasmid including Fas gene was transfected into Tca8113cell by lipofectamine kit.The expression of Fas mRNA in transfected and non-transfected cell was detected by RT-PCR.The expression of Fas protein and apoptosis index(AI)was assessed by flow cytometry(FCM).Results No variation of positive expression rate of Fas protein was found in Fas-transfected and non-transfected Tca8113(P>0.05),but the expression of Fas mRNA,intensity of Fas protein and apoptosis index were significantly increased respectively from0.201±0.015?31.02±4.63?0.88%±0.16%to0.399±0.073,54.32±6.38and10.21%±1.46%(P<0.01).Conclusion Apoptosis in tongue squamous cell carcinoma was not directly associated with the positive expression rate but intensity of Fas protein.A liminal value might exist before apoptosis in Tca8113cell could be activated.<