金银花提取条件对绿原酸含量的影响

Influeuce of extraction process on the content of Chlorogenic Acid in Flos Lonnicerae

目的 研究金银花中有效成分绿原酸的最佳提取条件。方法 采用正交实验法以乙醇浓度 (A)、溶剂量 (B)、提取时间(C)和提取次数 (D) 4个因素 ,每个因素选取 3个水平进行实验。结果 因素A ,因素D对绿原酸的含量有极显著影响。结论 以 1 0倍量 70 %乙醇为溶剂 ,85℃提取两次 ,每次

OBJECTIVE To optimize the extraction process of Chlorogenic acid from Flos Lonicerae. METHOD The optimum extraction was investigated by the orthogonal design and Chlorogenic acid determined by HPLC, the concentration of ethanol, volume of solvent, extraction hour and extraction times were the four factors in the experiment, and three levels of which were chosen to be inspected to optimize the process.RESULTS The concentration of ethanol and extraction times have significance effect on the content of Chlorogenic acid.CONCLUSION The best extraction process is extracting twice with decuple EtOH(70%) and refluxing at 85℃ for 1h each time.