不同剂量环磷酰胺对正常小鼠淋巴细胞增殖反应的影响

EFFECT OF CYCLOPHOSPHAMIDE ON THE ABILITY OF LYMPHOCYTES PROLIFE RATION IN NORMAL MICE

目的 :观察环磷酰胺对正常小鼠脾淋巴细胞转化和胸腺细胞自发掺入的影响。 方法 :采用3H_TdR掺入法。 结果 :10 -8和10 -7mol/L的环磷酰胺促进健康小鼠脾淋巴细胞转化 ,而≥10 -3mol/L的环磷酰胺抑制健康小鼠脾淋巴细胞转化 ;10 -3mol/L的环磷酰胺促进健康小鼠胸腺细胞自发掺入 ,而≥10-2 mol/L的环磷酰胺 ,则抑制健康小鼠胸腺细胞自发掺入。 结论 :大剂量环磷酰胺抑制健康小鼠免疫功能 。

Purpose: To observe the effect of cyclophosphamide(CP ) on the ability of splenic lymphocyte transformation and spon taneous proliferation of thymocytes in normal mice. Methods: 3H_ TdR incorporation technique was used. Results: The proliferation of splenic lymphocyte was significantly enhanced by CP in the concentration of 10-8 and 10-7 mol / L, but was markedly reduced by CP in the oncentration of more than 10-3 mol / L . CP in the concentration of 10-3 mol / L could 16.increase spontaneous proliferation of thymocyts, while CP in the concent ration of more than 10-2 mol / L inhibited it. Conclusion: La rge dose of CP inhibited immune function and low dose of C P enhanced it in normal mice.