TBARS的新应用—表征羰基紧张

Representing Carbonyl Stress: A New Application of TBARS

以硫代巴比妥酸(TBA)方法测得的所谓丙二醛(MDA)水平已被广泛用作诊断组织伤害和脂过氧化程度的指标。但TBA方法对MDA的特异性很有争议,MDA只是氧化伤害和脂质过氧化产生的诸多不饱和醛酮产物中主要产物的一种。由于硫代巴比妥酸反应产物(TBARS)涵盖了大部分氧化伤害产生的醛酮类物质,因而TBARS可被用作为衡量羰基紧张的很好的指标。

Malondialdehyde (MDA) level measured with thiobarbituric acid (TBA) method has been widely used as diagnostic indices of peroxidative tissue injury and lipid peroxidation. However, the specificity of the TBAtest toward MDA is disputed. MDA is merely one of the main unsaturated carbonyl products detected by TBA method. Since thiobarbituric acid reactive substances (TBARS) including most, if not all, of the unsaturated aldehydes/ketones resulted from oxidative stress, the using of TBARS may be useful as an index of carbonyl stress.