摘要
本文分别以人的肝癌细胞和动物脑组织为生物学对象 ,以荧光标记和同位素示踪为实验方法 ,以激光扫描共聚焦显微镜和液体闪烁计数器为检测仪器 ,以荧光强度和放射性强度为检测Ca2 + 量的特征指标 ,进行了电磁波生物学非热效应的典型代表电磁波生物学窗效应的实验研究。两种实验均表明了电磁波生物学频率窗效应和强度窗效应的存在且有相同的窗频率。基于本文的实验研究并结合其他文献的结果 ,可发现电磁波生物学窗效应具有以下基本特征 :(1)不同生物组织都会在 15~ 16Hz左右存在一个频率窗 ;(2 )生物学窗效应既可由ELF(极低频 )正弦 (调制波 )振幅调制的高频电磁波 (载波 )产生 ,又可由ELF连续波或ELF脉冲波产生 ,只是前者的频率窗只体现在调制波频率上而不体现在载波频率上 ,而且 ,前者的窗效应频率与后者的窗效应频率是相同的 ;(3)频率窗分布的一般规律为 fn=(2n +1) fc,式中 ,n =0 ,1,2… ,fn 为第n个窗频率 ,fc 为基频即最低的那个窗频率。实验研究还表明 ,这一分布规律在 0~ 135Hz范围内是正确的 ,且基频 fc≈ 15~ 16Hz;(4)只有特定频率参数与特定强度参数恰当组合的电磁波才能产生生物学窗效应 ,或者说 ,窗效应是电磁波频率和强度的二元函数。
A typical case of the athermal bio-effects of electromagnetic waves (EMW), the window bioeffects of EMW, was studied in this paper. The liver cancer cells of human being and the brain tissue of animals were investigated with the method of fluorescent mark and isotope tracing. The fluorescencence intensity and radioactivity intensity, which express the concentrations of Ca2+, were measured with the laser scanning confocal microscope and liquid scintillation counter. Based on the experimental results, it has been proved that there exists frequency-window and intensity-window bio-effects of EMW, and window frequencies from two experimental objects were the same. According to the conclusion of our research and the correlative references, the basic characters of window bio-effects of EMW are as fellows: (1) There exists an identical frequency window in different tissues, and the frequency is around 15-16Hz; (2) Either the amplitude modulation EMW or the ELF continuous and pulse EMW has the same results in producing the window bio-effect. The modulation waves have the same frequency with the ELF waves; (3) The distribution law of window effects is fn = (2n + 1)fc(n=0, 1,2), where, fn is the nth window frequency and fc is the basal frequency (the lowest window frequency), and the law is right in the frequency region 0 ∼ 135Hz, in which the basal frequency is 15 ∼ 16Hz; (4) The window bio-effects can only be produced in the specific frequency and specific intensity of EMW, in other words, the window effects is a dual function of the frequency and the intensity of the EMW.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2003年第2期126-132,共7页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目 (3 0 170 2 40 )
陕西省自然科学基金资助项目 (2 0 0 1X0 1)