摘要
以毛刺槐的花药为材料,开展其组织培养和植株再生系统的研究。结果显示:将毛刺槐的花药接种在MS附加2,4-D0.1mg/L和BA3.0mg/L的培养基上,20d时花药愈伤组织诱导率可达41.5％。花药愈伤组织在MS附加BA5.0mg/L的分化培养基上继代培养2个月后,可分化出许多绿色的芽点,待不定芽长至2～3cm高时将其切下,转入MS附加IBA1.0mg/L的生根培养基上,2周后即可得到完整的再生植株。同时,研究就4℃低温预处理和蔗糖浓度对毛刺槐花药培养的影响进行了研究和讨论。
The anthers of Robinia hispida were cultured and the results showed that the highest induction rate of callus was 41. 5% when they were cultured on MS medium supplemented with 2,4-D 0. 1 mg/L and BA 3. 0 mg/L for 20 days. Green buds formed from the callus after 2 months subcultured on MS medium supplemented with BA 5. 0 mg/L. When the shoots grew to 2-3 cm long,they were detached and trans-ferred to MS medium supplemented with IBA 1. 0 mg/L for rooting. The shoots produced roots within 2 weeks of culture and formed complete plantlets. At the same time,the effects of pretreatment of low tem-perature at 4 C and the concentration of sucrose on anther culture were studied and discussed.
出处
《西北植物学报》
CAS
CSCD
2003年第3期456-459,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
教育部重点基金
