美国黄松组织培养不定根诱导的研究

Study on root regeneration from adventitious buds in Pinus ponderosa

以GD、SH和1/2SH基本培养基对美国黄松不定芽进行不定根的诱导。试验结果表明基本培养基的种类对不定芽形成不定根起主要作用。在1/2SH培养基上附加0.5mg/L的NAA不定根的诱导率为3.3％。试验首次在离体培养条件下,以美国黄松种胚为外植体获得了再生小植株。

The adventitious buds of Pinus ponderosa were used to induce adventitious roots on GD,SH,1/2 SH media supplemented with different concentration of NAA and IAA. The results showed that the basie medium played a determinative role in initiating the adventitious roots. The better combination of the medi-um was 1/2 SH with 0. 5 mg/L NAA,on which 3. 3% adventitious buds formed adventitious roots. In the present paper,the experimental result is reported for the first time that root has been induced from cul-tured embryo of Pinus ponderosa.