鹅细小病毒NS2基因的克隆和序列分析

Cloning and sequencing of NS2 gene of goose parvovirus

本研究参考GenBank发表的GPVB株全基因序列 ,设计并合成一对引物 ,通过PCR技术扩增出GPVH1株NS2基因 ,目的片段长约 1.4kb ,将目的片段克隆到pMD18_T载体后进行序列测定和分析 ,结果表明 :GPVH1株NS2基因全长135 6bp ,编码 4 5 1个氨基酸 ,与国外已发表的GPVB株相比核苷酸序列同源性为 98.75 % ,推导氨基酸序列同源性为98.6 7%。

According to the sequence of GPV B strain published in GenBank, a pair of primers were designed by Oligo4.0.NS2 gene of GVP and amplified by PCR. The gene of interest was cloned into the vector pMD18_T and sequenced. The results showed that NS2 gene consisted of 1356 nucleotides and encoded 451 amino acids. The NS2 gene shared 98.75 % and 98.67 % homology with that of B isolate at nucleotide and amino acid level, respectively.