实验性禽脑脊髓炎中MIP-1β和IL-8的mRNA原位杂交实验

mRNA in situ hybridization of MIP-1β & IL-8 in experimental avian encephalomyelitis

禽脑脊髓炎病毒内蒙古地方毒株NH937株经脑内注射感染。于攻毒后 3,5 ,10 ,14 ,2 0 ,2 5 ,30d ,取攻毒组 4只 ,对照组 2只。取材大脑、小脑于 95 %乙醇中固定 ,经原位杂交染色 ,结果 :着染部位主要在大脑皮层锥体细胞及皮质下各核团的较大神经元的胞浆内 ,呈现蓝紫色连成片或单独数个较大颗粒 ,小脑蒲肯野细胞胞浆内也较多见 ;此外小胶质细胞胞浆内也发现很多呈阳性。在病情严重时的 10d开始增多 ,直到病情恢复的 30d时 ,仍然很普遍。相对于未攻毒的阴性对照组明显增多。说明禽脑脊髓炎过程中脑组织细胞内MIP_1β和IL_8的mRNA大量上调 ,主要与趋化T淋巴细胞到血管外形成管套 ,再从管套弥散向周围神经组织 ;

day_old SPF chickens were injected with AEV NH_937 via intracerebral inoculation and killed in 1,3,5,10,14,20,25,30th days postinjection, respectively. Collected their cerebrum and cerebellum, fixed and stained with in situ hybridization . The results showed there were many single or connected positive particles in the plasma of big awl cells and other big cells of cerebrum, and in Purkinje's cell in cerebullum. There were also many positive particles in plasma of many glial cells during 10～30th days postinjection. It showed that mRNA of IL_8 and MIP_1β were upregulated obviously in the process of avian encephalomyelitis. It means that the formation of perivascular cuffing and moving toward neural tissue, satellite appearance and engulfing degenerative and necrotic neurons all were caused by attracting of IL_8 and MIP_1.