大鼠出血性休克再灌注肾损伤与时间的关系

Correlation between time and renal injury reduced by hemorrhagic shock-reperfusion in rats

目的 探讨大鼠出血性休克再灌注后肾损伤与时间的关系。方法 采用修改的YU法制备大鼠模型 ,5 0只SD大鼠分为对照组、休克组、复苏 3h组、6h组、12h组、2 4h组、48h组 ,其中 8只大鼠按配伍组设计观察BUN及Scr的变化 ,42只大鼠按完全随机设计观察肾组织的常规病理和超微结构的改变。结果 大鼠休克末的平均失血量约占总血量的 60 42 %。再灌注 3h的BUN及Scr和 2 4h的肾组织病理损伤积分高于其他复苏组 (P <0 0 5 )。肾组织的超微结构变化也支持此结果。结论 再灌注后肾功能和结构损伤分别以 3h及 2 4h最明显 ;

Objective To study the correlation between time and renal injury reduced by hemorrhagic shock-reperfusion in rats. Methods The rats were bled to a mean arterial pressure(MAP) of 5 33 kPa, and maintained for 180 min by withdrawing more blood or returning little, and then were resuscitated with injection of sodium chloride, Fifty Sprague-Dawley Rats were divided into seven groups including the normal control, shock, and 3 h(SR 3),6 h,12 h,24 h(SR 24 ) and 48 h after resuscitation. BUN and Scr were measured in groups of 8 rats. Renal pathology was studied under light and electric microscope in 42 rats. Results The average blood loss by the end of shock was 60 42% of all blood volume in rat. BUN,Scr of SR 3 and pathologic score of SR 24 were the highest the groups of reperfusion( P <0 05), The change of renal ultrastructure was also the most severe. Conclusion Severe renal function domage and pathological lesion were seen at 3h and 24h respectively among the groups of reperfusion. Functional recovery is earlier than structural recovery.