抗视网膜母细胞瘤细胞株SO-Rb_(50)单克隆抗体相应抗原的提取

Separation of SO-Rb_ (50)cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma

目的 从 SO- Rb50 细胞中提取出抗视网膜母细胞瘤单克隆抗体相应的抗原。 方法 用离子交换层析法从 SO- Rb50 细胞中初步分离抗原 ,并利用抗视网膜母细胞瘤单克隆抗体以 dot blotting鉴定抗原 ,然后以十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳分离纯化出抗原目的蛋白条带 ,用 Westein blotting检测其相对分子质量。 结果 从 SO- Rb50 细胞裂解液的总蛋白中分离出一条抗原目的蛋白条带 ,其相对分子质量约为 83× 10 3。 结论 从 SO- Rb50 细胞裂解液中能够提取出抗视网膜母细胞瘤单克隆抗体对应的抗原。

Objective To separate the SO-Rb 50cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Methods The antigen corresponding to the monoclonal antibody of anti-retinoblastoma was separated elementarily by ion-exchange chromatography, and was identified by dot-blotting using the monoclonal antibody of anti-retinoblastoma. The target protein band of the antigen was separated in light of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results A special unmixed band of SO-Rb 50cells antigen was separated with the relative molecular weight of 83×103. Conclusion The antigen corresponding to the monoclonal antibody of anti-retinoblastoma could be separated from SO-Rb 50cells.