PKC及PKCI的活性与膀胱癌分期分级关系

Study on relationship between the activity of PKC, PKCI and the patho-logical grading, clinical staging in bladder transitional cell carcinoma

目的 :探讨蛋白激酶C(PKC)及其内源性抑制物 (PKCI)活性对膀胱癌发生发展的作用及其分子生物学机制。方法 :采用Takai法检测 38例膀胱移行细胞癌组织及 2 0例肿瘤周围正常组织胞质、胞膜PKC及PKCI的活性。结果 :膀胱移行细胞癌组织中 ,随病理分级增高 ,胞质、胞膜及总体PKC活性明显升高 (P <0 .0 5 ) ,胞膜 /胞质与胞膜 /总体PKC比率明显增大 (P <0 .0 5 ) ,胞质与总体PKCI活性明显升高 (P <0 .0 5 ) ,而胞膜PKCI活性却无明显变化 (P >0 .0 5 ) ,且胞膜 /胞质与胞膜 /总体PKCI比率明显减小 (P <0 .0 5 ) ;膀胱移行细胞癌组织中 ,随临床分期的增高 ,胞质、胞膜及总体PKC活性显著升高 (P <0 .0 1) ,胞膜 /胞质与胞膜 /总体PKC比率显著增大 (P <0 .0 1) ,胞质与总体PKCI活性显著升高 (P <0 .0 1) ,而胞膜PKCI却无明显变化 (P >0 .0 5 ) ,且胞膜 /胞质与胞膜 /总体PKCI比率明显减小 (P <0 .0 5 )。结论 :PKC及PKCI的活性变化与膀胱癌的病理分级、临床分期关系密切 ,PKC及PKCI活性膜质比或可成为评价肿瘤恶性程度及预后的指标。

Purpose:To investigate the effect of the activity of PKC and its inhibitor (PKCI) on the carcinogenesis of bladder transitional cell carcinoma and its molecular biological mechanism.Methods:Takai method was followed to detect the activity of PKC and PKCI in membranous and plasmic fraction of 38 cases of bladder transitional cell carcinoma, and 20 cases of the normal tissues three centimeter apart from the tumors.Results:Accompanying with the alteration of pathological grading of bladder transitional cell carcinoma, the activity of PKC increased in the plasmic and membranous fraction and total tissues, increased in the R M/P and R M/T (P< 0.05 ); the activity of PKCI increased in the plasmic and total tissues(P< 0.05 ), but not membranous fraction (P> 0.05 ), and the R M/P and R M/T of PKCI both decreased (P< 0.05 ). The relationship between the activity of PKC and PKCI and the clinical staging of bladder cancer was similar with that of the pathological grading.Conclusion:There was a close relationship between the activity of PKC and PKCI and the pathological grading level and clinical staging.in bladder transitional cell carcinoma.