摘要
目的 检测血管内皮细胞 (EC)缺氧 /再给氧反应后E selectin的表达 ,探讨抗氧化剂PDTC对血管内皮细胞E selectin转录的抑制作用。方法 以人胚肾血管内皮细胞为材料 ,建立缺氧 /再给氧模型 ,检测E selectin的表达 ;应用PDTC干预EC的缺氧培养过程 ,观察E selectin表达的改变。结果 血管内皮细胞缺氧 /再给氧反应后E selectin的表达明显增加 ,4h到达峰值 ;PDTC能有效抑制E selectin的表达。结论 缺氧 /再给氧促进EC活化 ,增强E selectin的表达 ,抗氧化剂PDTC能有效降低E selectin的转录 ,为缺血再灌注损伤的治疗提供理论基础。
Objective To measure the expression of E-selectin on hypoxia/reoxygenation-stimulated endothelial cells and investigate the effect on suppression of E-selectin by Pyrrolidine Dithiocarbamate.Methods Endothelial cells were exposed in hypxia condition and then returned to reoxygenation condition.ELISA method was used to detect the expression of E-selectin.Results E-selectin expression on hypoxia/reoxygenation-stimulated endothelial cells increased,and PDTC could suppressed translation of E-selectin effectively.Conclusion It seems that E-selectin may act as a critical factor.PDTC may prove beneficial in the treatment of ischemia/reperfusion injury.
出处
《四川医学》
CAS
2003年第5期450-451,共2页
Sichuan Medical Journal