河北省磁县食管癌高发区食管鳞状细胞癌组织HPV检测及FHIT表达的研究

HPV Detection and FHIT Expression in Esophageal Squamous Carcinoma from High Incidence Area in Cixian County

背景与目的:河北省磁县是食管癌高发区,有关人乳头状瘤病毒(humanpapillomavirus,HPV)感染和当地食管癌发生的关系尚无研究报道。本研究旨在探讨HPV感染在当地食管癌发生中的病因学意义,同时分析与环境致癌物密切相关的脆性组氨酸三联体基因(fragilehistidinetriad,FHIT)表达与HPV感染的关系。方法:收集河北省磁县食管癌高发区128例和非高发区24例食管鳞状细胞癌石蜡包埋组织,用PCR进行HPV检测,用免疫组化方法分析FHIT在蛋白水平上的表达情况,同时分析HPV感染和FHIT异常表达的关系。结果:PCR检测结果表明,河北省食管癌高发区食管鳞状细胞癌组织中HPV检出率为20.3%,略高于非高发区(8.3%),但两者差别无显著性(P>0.05)。免疫组化结果显示,食管癌高发区食管鳞癌组织中FHIT基因异常表达率(75.6%)明显高于非高发区食管鳞癌组织(54.2%,P<0.05)。食管癌组织中HPV感染和FHIT的异常表达之间无明显相关性。结论:河北省食管癌高发区食管癌组织FHIT的异常表达率明显高于非高发区病例。

BACKGROUND &OBJECTIVE: Cixian County is one of the high incidence areas of esophageal carcinoma in China. Up to now, no work has been conducted on the possible etiological role of human papilloma virus (HPV) infection on esophageal carcinoma in this local area. The aim of this study was to explore the putative roles of HPV infection on the esophageal carcinogenesis of the patients in Cixian and to elucidate the possible relationship between HPV existence and fragile histidine triad gene (FHIT gene, a putative tumor suppressor gene), expression in squamous cell carcinoma of esophagus. METHODS: The existence of HPV DNA and the expression of FHIT gene at protein level in esophageal carcinoma tissues were determined with PCR and immunohistochemical staining, respectively, in 128 archival paraffin embedded tissue blocks of esophageal squamous carcinoma from the high incidence area of Cixian and 24 tissue blocks from the non high incidence area. RESULTS: PCR results showed that the positive detection rate of HPV in esophageal carcinoma tissues from the high incidence area was 20.3%, which was slightly higher than that from the non high incidence area (8.3%)(P >0.05). Immunohistochemically, aberrant expression of FHIT gene in esophageal carcinoma tissue was found in 75.6%cases from the high incidence area and only 54.2%from the non high incidence area, which was significantly lower than that of the former (P< 0.05). No correlation was found between the abnormal expression of FHIT and existence of HPV DNA in esophageal squamous carcinoma tissues. CONCULSION: HPV DNA could be detected in partial esophageal carcinoma cases from the high incidence area of esophageal carcinoma in Cixian. The aberrant expression rate of FHIT protein in the cases from the high incidence area of esophageal carcinoma in Cixian is higher than that from the non high incidence area.