蛋白质组学技术识别Maspin在支气管上皮永生化细胞和恶性转化细胞中的差异表达

Proteomics Based Identification of Maspin Differential Expression in Bronchial Epithelial Immortalized Cells and Malignant Transformation Cells

背景与目的:Maspin蛋白是一种抑制肿瘤的丝氨酸蛋白酶抑制剂,它在抑制肿瘤生长和转移中发挥一定作用。本研究是利用蛋白质学技术鉴定Maspin在支气管上皮细胞恶性转化过程中的差异表达。方法:利用固相pH梯度等电聚焦双向电泳、肽质量指纹谱技术以及生物信息学技术,对Maspin在永生化细胞系及恶性转化细胞系的表达进行功能蛋白组学分析。结果:在分子量14.4～94kDa、等电点PI3～10范围内,2-D电泳分离出1500多个蛋白质点。凝胶图像分析显示Maspin蛋白在永生化细胞中的表达比恶性转化细胞表达高。Northernblot证实永生化细胞的MaspinmRNA丰度高于恶性转化细胞。结论:肺癌组织中Maspin在细胞转录和翻译水平的表达异常,这可能是肺癌发生的原因之一。

BACKGROUND &OBJECTIVE: Maspin,a serepin inhibitor, plays a key role in tumor growth and metastasis. The aim of this study was to identify the differential expression of Maspin in malignant transformation process of bronchial epithelial cells by proteomics. METHODS: Functional proteomics analysis of Maspin on bronchial epithelial immortalized cells and malignant transformation cells was carried out using immobilized pH gradient (IPG) two dimensional electrophoresis, peptide mass fingerprinting (PMF), and post source decay (PSD) of bio mass spectrometry. RESULTS:Nearly 1500 expressed proteins profile on bronchial epithelial immortalized cells and malignant transformation cells were obtained in the range of MW 14.4 94 kDa, PI 3 10. Image analysis showed that Maspin was down regulated in malignant transformation cells compared with that in immortalized cells. Northern blot analysis showed that the mRNA abundance of Maspin in malignant transformation cells was much lower than that in immortalized cells. CONCLUSION: Alteration expression of Maspin at transcription and translation levels might be involved in carcinogenesis of lung.