非小细胞肺癌E-钙粘蛋白基因异常的研究

The study of E-cadherin gene abnormality in non-small cell lung cancer

目的 观察非小细胞肺癌组织中E 钙粘蛋白 (E cadherin)的基因异常。方法 采用PCR SSCP及序列分析 ,研究了 2 4例非小细胞患者肺癌组织中E cadherin基因外显子 6、7、8、9的基因突变。结果 2 4例非小细胞肺癌中发现 2例存在E cadherinexon9的基因插入、缺失及突变。结论 E cadherin在非小细胞肺癌中存在基因异常改变 ,尤其集中在E cadherin的细胞外区域 ,主要表现为插入及缺失 。

Objective To observe the E cadherin gene abnormality in non small cell lung cancer(NSCLC). Methods E cadherin gene mutations in exons 6, 7, 8 and 9 in 24 cases of non small cell lung cancer were studied by PCR SSCP and DNA sequence analysis. Results Abnormality of the E cadherin gene was found in exon 9 by means of insertion, deletion and mutation in 2 cases. Conclusion Abnormality of E cadherin gene is present in NSCLC by means of insertion and deletion in extracelluar matrix. The main cause of the decrease in epithelial adhesion may be resulted from the abnormality gene change of the E cadherin.