摘要
目的 :建立反相高效液相色谱测定水杨酸血药浓度的方法 ,并将其用于阿司匹林复方制剂中水杨酸药代动力学及生物利用度的研究。方法 :采用Waters2690高效液相色谱仪 ,以DiamonsilC18 为色谱柱 (4 6mm×250mm ,5μm) ,以甲醇 -乙腈 -0 2 %磷酸 (18∶32∶50)为流动相 ,检测波长为237nm ,流速为1 0ml/min。结果 :水杨酸的浓度在0 40～101 00μg/ml范围内线性关系良好 (r=0 9999)。结论 :本法操作简便、灵敏、快速 ,适用于水杨酸的血药浓度测定及药代动力学研究。
OBJECTIVE:To develop a RP-HPLC method for the determination of salicylic acid in serum and to apply this method to the pharmacokinetic and bioavailability study of salycylic acid in compound aspirin preparations.METHODS:Waters2690HPLC instrument was used with Diamonsil C 18 column(5μm,250mm×4.6mm)as stationary phase and methanol-aceˉtonitrile-0.2%phosphoric acid(18∶32∶50)as mobile phase at a flow rate of1.0ml/min,and the detective wavelength was237nm.RESULTS:Calibration curve of salicylic acid was linear in the range of0.40～101.00μg/ml(r=0.9999).CONCLUS_ ION:The method is simple,sensitive,rapid and suitable for pharmacokinetic and bioavailability study of salicylic acid.
出处
《中国药房》
CAS
CSCD
2003年第5期289-291,共3页
China Pharmacy
