青藤碱对肾移植大鼠IL-2的影响

EFFECTS OF SINOMENINE ON IL-2 IN RAT RENAL ALLOGRAFT TRANSPLANTATION MODEL

目的 :研究青藤碱 (sinomenine,SIN)对肾移植大鼠存活时间及IL - 2的影响 ,探讨SIN免疫抑制作用的可能机制。方法 :实验分四个组 ,采用改良式大鼠肾移植术行Wistar→SD大鼠单肾移植 ,观测术后受体鼠的存活时间 ;ELISA法检测受体鼠外周血IL - 2水平。结果 :对照组受体鼠均在术后第 9天内死亡 ,平均存活时间 7.4± 0 .7d ,SIN组存活时间为 9.1± 1.0d ,而与低剂量CsA(2 .5mg/kg·d ,ip)联用后明显延长至 18d以上。SIN组受体鼠外周血IL - 2水平低于对照组 ,而SIN +CsA组受体鼠IL - 2水平明显低于对照组。结论 :SIN对大鼠肾移植的急性排斥反应具有一定的抑制作用 ,并与低剂量CsA产生显著的协同效应。SIN免疫抑制作用机制可能是抑制Th1细胞产生IL - 2 ,与CsA的作用机制相似。

Objective:To determine the effects of sinomenine on survival time and IL-2 in Rat Renal Allograft Transplantation Model. Methods:Kidney transplantations were performed from inbred Wistar rats to inbred SD rats (allogeneic) using the same modified technique described by Zhang zhiguo, et al. Experimental rats were divided into four groups. Group I as controls were not treated with medicine, the others received SIN, CsA and SIN combined with CsA respectively. The allograft survival time was observed and IL-2 levels of allograft were detected by ELISA mothed.Results:The mean survival time(MST) of group SIN was significantly increased and SIN combined with low-dose CsA was more effective than SIN alone. The level of IL-2 in group SIN and SIN+CsA was significantly decreased compared with control group. Conclusions:SIN can prolong the survival time of allograft and decline the level of IL-2 in rats, and SIN combined with low-dose CsA is more effective than SIN alone.