摘要
利用由粳稻品种Asominori与籼稻品种IR2 4的杂交组合衍生的重组自交F1 0 家系 (RecombinantInbredLines,RIL)群体及其衍生的染色体片段置换系 (ChromosomeSegmentSubstitutionLines,CSSL)群体 ,进行了种子休眠性QTL的检测和遗传效应分析。其中CSSL群体有 2个 ,即CSSL1(以Asominori为背景 ,置换片段来自IR2 4)和CSSL2 (以IR2 4为背景 ,置换片段来自Asominori)。在RIL群体上共检测到 3个种子休眠性QTL ,分别位于第 3、6和 9染色体上 ;在CSSL1群体中检测到分布在第 1、3和 7染色体上的 3个休眠性QTL ;而在CSSL2群体上检测到的 3个QTL则分别位于第 1、2和 7染色体上。同时在两套CSSL群体上 ,分别检测到位于第 1、7染色体上位置相近且效应一致的休眠性QTL ,分析表明其所在的Asominori片段含对种子休眠性的增效基因 ,相应的IR2 4片段含有减效基因。
A recombinant inbred line (RIL) population and two chromosome segment substitution line (CSSL) population derived from the cross of Asominori (japonica) and IR24 (indica) were used to detect QTL controlling seed dormancy.CSSL1 were a series of IR24 chromosome segment substitution lines in Asominori background,and CSSL2 were a series of introgression lines of Asominori in the background of IR24.Three QTL were detected on chromosome 3,6 and 9 in RIL population,and individual QTL accounted for between 12.3% and 13%.Three QTL were detected on chromosome 1,3 and 7 in CSSL1,and individual QTL accounted for between 11.5% and 18.9%.Three QTL were detected on chromosome 1,2 and 7 in CSSL2,and individual QTL accounted for between 11% and 16%.The QTLs on chromosome 1 and 7 were detected in CSSL1 and CSSL2 populations simultaneously,QTL came from Asomonori, the moderate dormant cultivar,increased seed dormancy,and QTL from IR24,the weakly dormant cultivar,decreased seed dormancy.It can be deduced that there exist genes controlling seed dormancy at this region.
基金
农业部 948项目 ( 2 0 10 0 2 (A) )
跨越计划项目 ( 1990 0 1 12 )
江苏省攻关项目 (BE2 0 0 130 5 )
省三项工程等项目的资助~~
