甘蓝型油菜pol CMS育性恢复基因对orf224/atp6的转录调控(英文)

Transcriptional Control of orf224/atp6 by the pol CMS Restorer Rfp Gene in Brassica napus L

用 10个线粒体基因探针对波里马细胞质雄性不育 (polimaCMS)三系 1141A(pol) ,1141B(nap)和 1141R(pol)的花蕾线粒体RNA进行了Northern检测。结果表明 ,只有 3个探针atp6、orf2 2 4和orf2 2 2检测到转录本的差异。atp6在可育的 1141B中只转录产生一个丰度很高的 1 1kb转录本 ,在雄性不育的 1141A和pol胞质恢复系 1141R中 ,这个转录本的丰度明显减少并出现了分子量较大的 2个转录本 2 2kb、1 9kb转录本。与 1141A相比 ,恢复系1141R的 2 2kb和 1 9kb转录本丰度明显减少 ,并伴随着两个新的转录本 1 4kb和 1 3kb。表明orf2 2 4 atp6的表达与polCMS有关 ,并且其转录受到恢复基因Rfp的调控。同时通过对杂种F1 ( 1141A× 1141R)与另一个恢复系RS35 (pol)的比较证实 ,Rfp对orf2 2 4 atp6的调控与Rfp纯合与否无关。orf2 2 4 atp6在 1141A的苗期叶片中还转录产生育性恢复特异的 1 4kb转录本 ,这可能与细胞核基因型和相对低温条件有关。

In order to investigate effects of fertility restorer gene Rfp on expression of orf224/atp6,Northern analyses of floral mitochondrial RNA (mtRNA) from polima (pol) CMS three lines were conducted using 10 probes of mitochondrial genes in this paper.Differences in transcriptional patterns were observed only with the probes atp6,orf224 and orf222.The atp6 probe detected a single,1.1 kb transcript with strong abundance in the male fertile 1141B (nap cytoplasm).In the male sterile 1141A (pol cytoplasm) and fertile restorer line 1141R (pol cytoplasm),levels of this transcript appeared greatly reduced,and two longer transcripts of 2.2 and 1.9 kb were observed.Compared with 1141A,levels of the 2.2 and 1.9 kb transcripts appeared to be slightly reduced in the restorer line 1141R,and two additional transcripts of 1.3 and 1.4 kb were observed.The results suggested that the expression of orf224/atp6 gene region was associated with pol CMS and regulated by Rfp gene.In contrast to floral buds of the male sterile 1141A,the 1.4 kb transcript specific to fertility-restored plants was also observed in seedlings of 1141A.Possible mechanisms by which transcriptional alteration of orf224/atp6 between floral buds and seedlings of 1141A may be related to nuclear genotypes and relatively low temperature are discussed.