丽江山慈姑的组织培养及育种技术研究

Studies on tissue culture and rapid propagation technique of Iphigenia indica

目的 建立药用植物丽江山慈姑的组织培养和快速繁殖体系。方法 对丽江山慈姑的球茎、地上茎、叶片及根尖等不同部位进行丛生芽及原球茎的诱导 ,筛选适合生根的培养基配方。结果 通过研究 ,筛选出了丽江山慈姑原球茎的诱导、增殖及生根培养基配方 ,建立了丽江山慈姑的快速繁殖体系 ,实现了大规模的组培生产。结论 在MS培养基上 ,丽江山慈姑球茎和地上茎诱导丛生芽效果较好 ,MSF 6 BA 2mg/L +NAA 0 5mg/L的激素配比既能使丽江山慈姑快速增殖 ,又能诱导出原球茎 ,适合大规模生产 ,在 1/ 2MS +IBA 0 5mg/L +NAA 0 2mg/L的培养基上 ,丽江山慈姑原球茎生根效果较好。

Object To establish a tissue culture and rapid propagation system for the medicinal plant Iphigenia indica Kunth. Methods Clusters of seedlings and protocorms and induction were studied on MS media with different parts of the plant, such as corms, stems, leaves and root tips as explants. Results The proper media for protocorm inducing, propagation and rooting were found in this paper, rapid propagation system of I. indica was established and the plant could put in large scale production. Conclusion Sprout can be successfully induced from corm and stem on MS media. MS+6-BA 2 mg/L+NAA 0.5 mg/L can induce both sprout and protocorm, and fit for large scale production. 1/2 MS+IBA 0.5 mg/L+NAA 0.2 mg/L is good for rooting.