摘要
目的 探讨Survivin基因在人脐血CD34 +干 /祖细胞中的表达及意义。方法 采用链霉亲和素 生物素 过氧化酶 (SABC)技术和RT PCR技术对人脐血CD34 +细胞中Survivin蛋白及mRNA进行了检测。结果 Survivin基因在新鲜脐血CD34 +细胞中有表达 ,蛋白表达率为 9.4± 1.2 ,SurvivinmRNA为弱阳性。在体外培养过程中 ,造血生长因子可上调Survivin基因的表达 ,其中以SCF、FL、Tpo组合作用最显著 ,体外培养 3d后 ,Survivin蛋白表达率为 2 5 .2± 5 .3 ,SurvivinmRNA表达增强 ,去除细胞因子后 2 4hSurvivin表达下降。结论 Survivin基因不是癌细胞特异性抗凋亡蛋白 ,在正常人造血干 /祖细胞中有表达 ,对正常人造血过程有调节作用。
Objective To investigate the expression and significance of survivin protein and mRNA in cord blood(CB) CD 34+ stem/progenitor cells. Methods Survivin level in CB CD 34+ cells was assessed by immuno-histochemistry and reverse transcription polymerase chain reaction (RT-PCR). Results Survivin expressed in fresh CB CD 34+ cells, its protein level was 9.4±1.2, faint signal of the mRNA transcript band was detected. In the in vitro culture of the cells, hematopoietic growth factors could upregulate survivin expression, especially the combination of SCF, FL and Tpo. After 3 days culture, its protein level was 25.2±5.3, and mRNA transcript increased significantly. Survivin expression dropped down 24 hours after withdraw of these cytokines. Conclusion Survivin is not a specific anti-apoptotic protein of cancer, and also expressed in normal hematopoietic stem/progenitor cells. It plays important regulating roles in the process of hematopoiesis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2003年第5期238-240,共3页
Chinese Journal of Hematology
基金
国家自然科学基金海外青年学者合作研究基金资助项目 ( 3992 80 10 )
