钙通道拮抗剂增强内吗啡肽镇痛效应的研究

Potentiation of the antinociceptive effect of endomorphin-1 by verapamil in the rat

目的 :观察钙通道拮抗剂与内吗啡肽 1(endomorphin 1,EM 1)联合外周应用对大鼠的镇痛效应。方法 :痛阈测定选用电刺激鼠尾 -嘶叫法。脊髓蛛网膜下腔注射不同剂量的EM 1(12 .5 μg/kg ,2 5 μg/kg ,5 0 μg/kg)或维拉帕米 (verapamil,5 0 μg)加EM 1(12 .5 μg/kg ,2 5 μg/kg ,5 0 μg/kg)联合用药。注药 15min后测定痛阈。 结果 :EM 1单独应用时 ,镇痛效应确切 ,在所试剂量范围内与剂量有关。维拉帕米与不同剂量EM 1联用 ,可显著增强EM 1的镇痛效应。预注受体阻断剂Cyprodime的试验表明 :维拉帕米增强EM 1的镇痛作用与阿片受体有关。结论 :钙通道拮抗剂可增强EM

Objective:To investigate the effects of calcium channel blocker on the antinociception of endomorphin 1(EM 1) when the two drugs were combined intrathecal in rats. Methods:Tail stimulation vocalization test were used to assess nociceptive sensitivity. EM 1(12.5 μg/kg,25 μg/kg,50 μg/kg) or veraparmil(50 μg) and EM 1(12.5 μg/kg,25 μg/kg,50 μg/kg) were injected intrathecal.Results:Intrathecal administration of EM 1 induced a dose dependent antinociception. Verapamil could enhance the effect of EM 1, while pretreatment with cyprodime could partly abolish this synergistic effect. Conclusions:Calcium channel blocker could enhance the effect of EM 1. [