ras、p53基因表达与丙型肝炎病毒感染相关性的免疫组化研究

Immunohistochemical study on the correlation between HCV inf ection and expression of ras oncogene and tumor suppressor gene p53

目的 探讨丙型肝炎病毒 (HCV)感染与肝细胞癌 (HCC)之间的关系以及HCV可能的致癌机理。方法 采用免疫组织化学方法检测 72例肝细胞癌标本癌及癌周组织中的HCVNS3抗原、ras基因的P2 1及抑癌基因P5 3蛋白。结果 肝细胞癌及癌周肝组织中有HCVNS3抗原检出 ,支持HCV与HCC之间有关联。在HCC的癌及癌周组织中P2 1呈致密的过量表达 ,提示ras癌基因的激活在HCC的发生过程中起一定作用。P5 3的阳性率较P2 1低 ,但 p5 3的突变似乎也是肝癌发生的协同因素之一。组织中P2 1的过量表达与HCVNS3抗原阳性检出呈正相关。结论 HCV感染作为HCC的密切相关因素之一 。

Objective To elucidate the relationship betwe en HCV infection and HCC,and understand the possible carcinogenic mechanism of HC V.Methods HCVNS3 antigen,P21 and P53 in the liver tissues of 72 cases of HCC were detected with immunohistochemical method.Results HCVNS3 antigen could be discovered in the cancerous tis sues and/or pericancerous tissues of HCC,which suggests that HCC is associated with HCV infection.P21 overexpression was demonstrated in the cancerous and peri cancerous parts of most HCC specimens.This result indicates that activation of r as gene may play an important role in the oncogenesis of HCC.Though the incidenc e of P53 was comparatively lower than that of P21,mutation of P53 gene seems to be a cooperative factor in the oncogenesis of HCC.Expression of P21 and P53 wer e positively related to HCV NS3 antigen.Conclusions HCV infection may be one of the factors closely rel ated to HCC,which induces the malignant phenotypic alteration of hepatocytes th rough activating some oncogenes or mutating some tumor suppressor genes.