氮源对重组枯草芽孢杆菌24/pMX45核黄素发酵的影响

Influence of Different Nitrogen Sources on Riboflavin Fermentation

考察了无机氮源NH4 Cl和不同有机氮源对重组枯草芽孢杆菌 2 4 / pMX4 5核黄素发酵的影响 .实验结果表明 ,NH4 Cl作为基础氮源对菌体生长和核黄素合成均有抑制作用 .采用不同有机氮源进行核黄素发酵 ,以酵母粉为氮源时发酵单位最高 ,而蛋白胨为氮源时核黄素发酵单位最低 .

The influences of inorganic nitrogen source NH 4Cl and several organic nitrogen sources on riboflavin fermentation were studied. The results showed that NH 4Cl as the basic nitrogen source had a negative effect on the Bacillus subtilis growth and the riboflavin synthesis, while the organic nitrogen source had a positive effect on the growth and the riboflavin synthesis.The riboflavin production was high when yeast powder was used as the nitrogen source and was low when peptone used as the nitrogen source.