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HBc与HBV多表位复合基因在肝癌细胞中的转染与表达 被引量：1

Expression of the Multiepitope Gene of HBV in HepG2 Cells
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摘要 目的观察HBc与HBV多表位复合基因在肝癌细胞株nepG2细胞内表达情况。方法将构建的HBc与HBV多表位复合基因真核表达质粒pHBcMep以Lipofectamine介导转染HepG2细胞,通过间接免疫荧光、Western-blot检测表达产物。结果经G418筛选的阳性转染细胞经间接免疫荧光染色后在紫外光激发时发出明显的荧光,而未转染质粒或转染pcDNA3.1的HepG2细胞则无明显荧光。Western-hot结果显示表达产物约为33kDa,并能与抗preS2多抗特异性结合。结论HBc与HBV多表位复合基因在肝癌细胞内获得正确表达。 Objective To investigate the expression of the HBc and raultiepitope of HBV fusion protein in HepG2 cells. Methods The recombinant plasmid encoding HBc and multiepitope(Mep) of HBV was transfected into HepG2 cells with Iipofectamine,and the cell clones were obtained with G418 screening.The expressed HBc-Mep protein was detected by ELBA, immuofluorescence assay(IFA) and Western-blot. Results The results of ELBA, IFA and Wetem-blot indicated that the HBc-Mep fusion protein, about 33kDa was expressed in transfected HepG2 cells. Conclusion The chimera gene encoding HBc and multiepitope of HBV was expressed in HepG2 cells successfully. The recombinant plasmid could be a potential vaccine candiate.
作者 田泽维 董文其 刘朝霞 李明 黄建生
机构地区 第一军医大学热带病研究室 第一军医大学分校检验教研室
出处 《热带医学杂志》 CAS 2003年第1期25-27,共3页 Journal of Tropical Medicine
基金 国家自然基金资助(No.30170532) 广州市重点攻关课题
关键词 HBC HBV 多表位复合基因 肝癌 转染 表达 HBV HBc multiepitope eukaryotic expression HepG2
分类号 R735.7 [医药卫生—肿瘤]
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共引文献5

同被引文献12

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热带医学杂志
2003年 第1期

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