一步固化法制作胰岛移植微囊的实验研究

The experimental study on the microcapsules made by the method of one step solidification for islet transplantation

目的 采用一步固化法制作用于胰岛移植的海藻酸钠 -氯化钡微囊 ,研究这种微囊的牢固度及生物相容性。方法 用海藻酸钠和氯化钡为材料 ,采用一步固化法制成微囊 ,观察这种微囊的形态和大小。将其置于生理盐水中 37℃孵育 2个月或置于生理盐水中 37℃、15 0r min水浴振荡1h ,观察微囊的破损率及形态改变。将 5 0 0 0个空微囊分别注射于 12只大鼠腹腔内 ,于 2、6、10周各取 4只大鼠处死后用生理盐水灌洗腹腔 ,将灌洗液离心后计算其中空微囊数并观察微囊的形态改变。结果 一步固化法制作的海藻酸钠 -氯化钡微囊在 37℃培养箱孵育 2个月后破损率为 10 % ;于 37℃、15 0r min水浴振荡 1h后破损率为 18% ;植入空微囊后第 2周每只大鼠可灌洗出空微囊约 30 0 0个 ,微囊均呈圆形 ,完整 ,无纤维化 ;6 - 10周每只大鼠可洗出空微囊约 10 0 0 - 15 0 0个 ,其中 10 % -15 %微囊变形或有裂口 ,部分微囊有明显纤维化。结论 一步固化法制作的海藻酸钠

Objective To study the solidity and biocomatibility of the microcapsules made by the method of one step solidification. Methods Microcapsules for islet transplantation were made of alginate and BaCl 2 by the method of one step solidification. 2 000 hollow microcapsules were suspended in normal saline at 37 ℃ for 2 months; another 2 000 hollow microcapsules were put into normal saline at 37 ℃ and shaken at 150 r/min for 1 hour, the ratio of the broken microcapsules was counted and the changes of their shape were observed. 5 000 microcapsules per rat were injected into the peritoneal cavity of 12 Wistar rats, four rats were killed at 2, 6, 10 weeks respectively and their peritoneal cavities were washed out with saline, the ratio of the broken microcapsules was counted and the changes of their shape were observed. Results The ratio of the broken microcapsules were 10% and 18%. At 2nd week about 3 000 microcapsules per rat were washed out, and they were intact and round without fibrosis. At 6-10 weeks, only 1 000-1 500 microcapsules were washed from one rat, 10%-15% of them were deformed or broken with remarkable fibrosis. Conclusions The alginate-BaCl 2 microcapsules made by the method of one step solidification have good solidity and biocompatibility.