腺病毒DNA对肝细胞系的转化活性的研究

The Conversion Activation of Adenovirus DNA on a Hepatocyte Cell Line

用含有腺病毒EIA和EIB基因的DNA片段对一株由猴病毒40(SV_(40))转化的大鼠肝细胞系——CWSV-1细胞进行了转化实验,以了解腺病毒基因和SV_(40)DNA之间的关系。我们的结论是:(1)腺病毒DNA能转化CWSV-1细胞;(2)建立的NP细胞呈重叠生长趋势,当细胞复盖培养基达60％左右时,大部份细胞自行脱落,但细胞形态没有明显改变;(3)形成的NP细胞系大部份不再分泌可检测的白蛋白,少量细胞分泌白蛋白的能力也随着传代而消失。

The plasmid which carried adenovirus DNA was used to transform a low passage SV_(40)-immortal- ized hepatocyte cell line(CWSV-1)to find out the relations between the virus genes.We conclude. that:(1)CWSV-1 cells can be transformed by transfection with adenovirus DNA;(2)The trans- formed cells by adenovirus DNA(NP cells)grow in a overlapping way.The cells come off when they cover 60％ of dish;(3)The NP cells do not retain the ability to express detectable albumin.