摘要
以α-^(32)p-dCTP标记的C-MYC癌基因为探针,采用斑点杂交法检测38例慢性宫颈炎,49例宫颈癌组织DNA中C—MYC癌基因的同源序列。结果慢性宫颈炎、宫颈癌杂交阳性率分别为26.32%(10/38)、83.67%(41/49)。而以α-^(32)p-dCTP标记的C-MYC重组体作探针进行杂交,结果49例宫颈癌杂交阳性率分别为100%(49/49)。结果提示:(1)分子杂交法检测以C—MYC DNA的同源序列有助于宫颈癌的早期诊断;(2)人体组织基因组中存在有PBR_(322)的同源序列,因此以克隆化重组体作探针进行条交必须注意到载体所产生的假阳性。
We detected the analogous sequences of C-MYC oncogene to the uterine cervical tissue genome DNA by means of dot blot hybridization,It shows that the positive rates were 26.32%(10/38), 83.67%(41/19)respectively,in chronic cervititis and cervical carcinoma if we used the probe of C- MYC DNA labeied by α-^(32)P-dCTP,The positive rates of C-MYC gene were 100%(49/49)if we used the probe of C-MYC recombinant DNA labeled by α-^(32)P-dCTP.We concluded from all of the above mentioned results that:①Detecting the analogous sequences of C-MYC gene helps to diagnose the uter- ine cervical cancer earlier with hybridization.②There are analogous sequences of PBR_(322) in human genome.So it must attend to make psuedo-positive rate with probe of recombinant DNA.
基金
<七.五>国家科研攻关专题资金资助