隐孢子虫病患者与机体细胞免疫功能相关性的研究

Study of the relationship between the patients infected by Cryptosporidium par vum and the change of cellular immune function in their bodies

目的 探讨隐孢子虫病患者与机体细胞免疫功能的关系。方法 采用生物素 链霉亲和素 (Biotin Streptavidin,BSA)法对卵囊阳性患者分别进行外周血T细胞亚群、膜白介素 2受体 (mIL 2R)检测。结果 隐孢子虫卵囊阳性者的CD3+ 、CD4 + 、CD8+ 和CD4 + /CD8+ 阳性百分率分别为 (5 5 .87± 7.2 3) %、(39.2 6± 6 .4 3) %、(30 .0 4± 5 .6 7) %和 (1 .36± 0 .4 1 ) % ,诱导期mIL 2R表达水平为 (33.4 5± 2 .31 ) % ,两者分别与卵囊阴性者及静息期mIL 2R相比 ,差异有显著性 (P <0 .0 5～ 0 .0 1 )。结论 隐孢子虫病引起细胞免疫功能的变化 ,T细胞亚群、mIL

Objective To explore the relationship between the patient s infected by Cryptosporidium parvum and the change of cellular immune funct ion in their bodies. Methods Biotin Streptavidin (BSA) and ELISA w ere used to detect T subsets and membrane interleukin 2 receptor (mIL 2R) in p eripheral blood mononuclear cell (PBMC) of the patients infected by Cryptospor idium parvum respectively. Results The positive rates of CD3 +,CD4 +,CD8 + and CD4 +/CD8 + in the patients in whose stools Cry ptosporidium parvum oocyst was found were (55.87±7.23)%,(39.26±6.43)%,(30 .04±5.67)% and (1.36±0.41)% respectively , and the level of mIL 2R in induct ion period was (2.92±1.06)%. It could be concluded that the positive rates of CD3 +,CD4 +,CD8 + and CD4 +/CD8 + in the patients in whose stools Cryptos poridium parvum oocyst was found were significantly different from which i n the patients in whose stools Cryptosporidium parvum oocyst was not found( P <0.05-0.01). The level of mIL 2R in induction period was significantly differe nt from which in resting period ( P <0.05-0.01). Conclusions In the patients infected by Cryptosporidium parvum , cellular immune partic ipat e resisting the parasite , and T subsets and mIL 2R play an important role.