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获取全长cDNA若干方法的比较 被引量：2

Comparison of the Approaches for Full-length cDNA Enrichment
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摘要 Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed. Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed.
作者 陶爱林 林兴华 张端品
机构地区 华中农业大学作物遗传改良国家重点实验室
出处 《武汉植物学研究》 CSCD 2003年第2期179-186,共8页 Journal of Wuhan Botanical Research
基金 国家自然科学基金项目 (3 0 170 5 69) 国家"863"计划资助课题 (Z16-0 2 -0 1-0 2 )
关键词 全长CDNA 信使RNA 5′帽子结构 高保真PCR 比较 Full length cDNA mRNA 5′ CAP High fidelity PCR Comparison
分类号 Q943 [生物学—植物学]
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参考文献49

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同被引文献57

  • 1张新宇,高燕宁.PCR引物设计及软件使用技巧[J].生物信息学,2004,2(4):15-18. 被引量：102
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武汉植物学研究
2003年 第2期

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