L-精氨酸抑制高脂喂饲大鼠动脉核因子-κB的合成

Dietary L-Arginine Attenuates Expression of Nuclear Factor Kappa B in the Aorta of Hypercholesterolemic Rats

目的 :观察L 精氨酸对高脂喂饲大鼠动脉核因子 κB合成的影响。方法 :雄性Wistar大鼠 45只 ,随机分为 3组 (n均 =15 ) ,对照组 (普通饲料及饮水 ) ,胆固醇组 (高胆固醇饲料及饮水 ) ,精氨酸组 (高胆固醇饲料喂饲的同时饮水中含 3 %L 精氨酸 ) ;3组喂饲 12周后采动脉血 ,处死大鼠取腹主动脉全长利用WesternBlot检测核因子 κB的含量。结果 :精氨酸组及胆固醇组大鼠血清胆固醇浓度无明显差异 ,但高于对照组 (P <0 0 1) ,精氨酸组血清一氧化氮水平明显高于胆固醇组 (P <0 0 1) ,精氨酸组核因子 κB的合成与对照组无差异 ,但低于胆固醇组 (P <0 0 1)。结论 :L 精氨酸抑制大鼠动脉核因子 κB合成的作用可能是其抑制高脂膳食诱发的动脉粥样硬化的分子机制之一。

Objective: To investigate the effect of L-arginine on nuclear factor kappa B expression in the aorta of hypercholesterolemic rats. Methods:Forty five male Wastar rats were randomized into three groups(n=15).Group NC was given normal diet(NC, n=15), group CC hypercholesterolemic diet(CC,n=15) and group AC hypercholesterolemic diet supplemented with 3% L-arginine in drinking water(AC,n=15).After 12 weeks blood samples were collected for biochemical studies, and the aortas were harvested for Western Blot. Results:Serum cholesterol concentration in AC and CC groups had no significant difference(p>0.05), but it was significantly higher than that of the NC group(p<0.01).Serum nitric oxide concentration of the NC and AC groups had no significant difference(p>0.05),but it was significantly higher than that of the CC group (p<0.01).Dietary L-arginine supplementation(group AC) significantly reduced the expression of nuclear factor kappa B(p<0.01). Conclusions:Inhibitory effect of dietary L-arginine on nuclear factor kappa B expression may be one of the mechanisms of the anti-atherogenic effect of L-arginine.