热休克蛋白70在外阴白斑和外阴鳞癌中的表达及临床意义

Expression and clinical significance of heat shock protein 70 in genitalia leukoplakia and squamous cell carcinoma of vulva

目的 :研究热休克蛋白 70 (HSP70 )在外阴白斑和外阴鳞癌中的表达 ,探讨其在外阴白斑和外阴鳞癌发生、发展中的意义 .方法 :应用免疫组化法检测外阴白斑 80例 ,不典型增生 13例 ,外阴鳞癌 4 8例和正常皮肤组织 2 0例中HSP70的表达 .结果 :HSP70在外阴白斑各分型中阳性率分别为 :增生型 31.2 % (10 / 32 ) ,硬化萎缩型 9.1% (2 / 2 4 ) ,混合型 2 9.2 %(7/ 2 4 ) ,不典型增生型 4 6 .2 % (6 / 13) ;在正常皮肤组织中阳性率为 0 (0 / 2 0 ) ;在外阴鳞癌中阳性率为 6 2 .5 % (30 / 4 8) ,在鳞癌、外阴不典型增生、外阴白斑中HSP70的表达和正常组织表达率相比有非常显著差异 (P <0 .0 1) .HSP70在I级 ,II级 ,III级外阴鳞癌中阳性表达率分别为 5 5 .0 % (11/ 2 0 ) ,5 5 .6 % (10 / 18) ,90 .0 % (9/ 10 ) .其表达在鳞癌各组织分级之间比较无明显差异 (P >0 .0 5 ) .结论 :HSP70在外阴白斑、外阴不典型与鳞癌中的表达情况存在明显差别 .

AIM: To investigate the expression of HSP70 and to evaluate its clinical significance in the occurrence and development of genitalia leukoplakia and squamous cell carcinoma of vulva (SCC). METHODS: Using the method of immunohistochemical, human HSP70 was detected in 80 cases of genitalia leukoplakia, 13 cases of squamous cell carcinoma of vulva and 20 cases of normal skin. RESULTS : The positive rates of HSP70 in different types of genitalia were 31.2% in hyperplasia type, 9.1% in sclerotic type, 29.2% in mixed type, 46.2% in atypical hyperplasia and 0% in normal skin, respectively. The positive rate of HSP70 was 62.5% in patients with SCC, which was significantly higher than that of genitalia leukoplakia type and normal skin ( P <0.01). The positive rates of HSP70 in the different grades of SCC were 55.0% (11/20) in grade I, 55.6% (10/18) in grade II and 90.0% (9/10) in grade III ( P >0.05). CONCLUSION: The results suggest that HSP70 expression is different in different tissues and the aberrant expression of HSP70 is closely related to the tumorgenesis and progression of SCC.