胆红素脑病仔兔黑质-纹状体系神经元计数及超微结构的研究

Study of ultrastructure and neuron quantitative determination in nigra-striatum of newborn rabbits with bilirubin encephalopathy

目的探讨胆红素对黑质 纹状体系神经元的毒性作用及损伤程度。方法 30只纯系日产大耳白仔兔随机分为 3组 :对照组 (C组 ,6只 )腹腔注射生理盐水 1ml ,注射后 6小时灌杀 ;模型 1组 (N1组 ,12只 )腹腔注射胆红素 10 0mg/kg ,分别于给药后 6小时 (N1a组 ,6只 )、16小时 (N1b组 ,6只 )灌杀 ;模型 2组 (N2 组 ,12只 )腹腔注射胆红素 2 0 0mg/kg ,分别于给药后 6小时 (N2a组 ,6只 )、16小时 (N2b组 ,6只 )灌杀。用图像分析仪进行细胞计数 ,电镜分析超微结构变化。结果N1b组黑质神经元数少于C组、N1a组(P <0 .0 5 ) ,N2b组黑质神经元数明显少于N1b、N2a组 (P <0 .0 5 )的黑质 纹状体系神经元数量减少 ,神经元超微结构有不同程度的改变。结论胆红素能减少黑质 纹状体系神经元数量 ,且剂量越大、时间越长 ,减少越明显 ;胆红素脑病时黑质、纹状体系神经元超微结构改变明显 ,且剂量越大、时间越长损伤越重 ;黑质、纹状体系的形态。

ObjectiveTo explore the noxious effect and the extent of damage in nigra-striatum neuron of bilirubin.Methods30 newborn rabbits were divided into 3 groups randomly:control group(group C,12 rabbits) which were injected normal salt intraperitoneally,model group 1(group N 1,12 rabbits) which were injected bilirubin 100mg/kg intraperitoneally, model group 2(group N 2,12 rabbits) which were injected bilirubin 200mg/kg intraperitoneally. All the rabbits in group C and 6 rabbits in group N1(group N 1a) and group 2(group N 2a) were killed 6 hours after injection.Other 6 rabbits in group N1(group N 1b) and group 2(group N 2b) were killed 16 hours after injection. The quantity of neuron in nigra-striatum were determined,and the changes of ultrastructure were observed by electron microscope.ResultsThe neuron in nigra-striatum in group N 2b were decreased compared with group C and group N 1a(P<0.05 ),as well as with group N 1b and N 2a. The ultrastructure of the neuron was changed.ConclusionsThe changes in utrastructure and the quantity of nigra-striatum neuron were associated with the concentration and time of exposure under bilirubin. It is consisted with the changes of ultrastructrue and quantity in nigra-striatum.