犬2型腺病毒E1转化细胞系的特性研究

THE STUDIES ON CAV-2 SY STRAIN E1 TRANSFORMED CELL LINE

对筛选到的一株CAV-2 E1转化细胞(DK/E1)进行了初步的特性研究。以DK细胞为对照,观察到转化细胞的形态与DK细胞有明显区别,细胞变长,易聚集生长。通过测定DK及DK/E1细胞分别在2％、5％和10％牛血清培养基中的生长曲线,结果发现DK/E1细胞的生长速度比DK细胞快。用蚀斑和TCID50测定了病毒在DK和DK/E1细胞上的病毒滴度,结果表明DK/E1细胞产生的病毒蚀斑比DK细胞的大,TCID50测得的病毒滴度比DK细胞产生的病毒滴度高。CAV-2全基因组DNA对DK/E1细胞和DK细胞的转染试验结果表明,5μg和10μg的CAV-2DNA转染的DK/E1细胞,分别在转染后第14天和第11天均出现了典型的腺病毒细胞病变,而相同量CAV-2 DNA转染的DK细胞未出现病变,说明DK/E1细胞有助于CAV-2 DNA的复制和病毒粒子的包装。在DK/E1细胞内的重组试验表明,DK/E1细胞也有利于重组病毒的产生。以上转化特性在DK/E1细胞传至80代时仍保持不变,说明本试验获得了一株CAV-2 E1基因的转化细胞系。

Parts of the properties of the CAV-2 El transformed cell line (DK/E1) were studied in this paper. Compare with the normal DK cell line, the DK/E1 cell line has parts of the properties of transformed cell, such as cell becames longer, grows faster, nutrien require decreases, et al. Our experiments also found that the virus titer produced on DK/E1 cell is higher than that on DK cell. The DK/E1 cell can promote the replication of the CAV-2 DNA and the package of the virus particle, even the production of recombinant virus. The properties of transformed cell of the DK/E1 can subculture for more than 80 generations.