特异性下调GRP94对人大肠癌细胞生物学特性的影响

THE EFFECT OF SPECIALLY DOWN-REGULATED GRP94 ON THE BIOLOGICAL PROPERTIES OF HUMAN COLORECTAL CANCER CELL LINE

为观察GRP94对培养的人大肠癌细胞系CCL229生物学特性的影响,将特异性裂解GRP94 mRNA翻译起始区的核酶,用脂质体介导的转染方法导入培养的CCL229细胞中。在确定获得稳定转染株后,我们检测了细胞生物学特性的变化。结果为:(1)转染GRP94核酶的细胞在A23187诱导16h后,细胞生长率显著降低。(2)核酶表达细胞诱导后的聚集能力明显下降。(3)核酶表达细胞在A23187诱导后,停滞在G0/G1期的比例明显升高。结论为CRP94与应激的大肠癌细胞的生长和侵袭能力密切相关;特异性下调GRP94能改变人大肠癌细胞系CCL229的一些生物学特性。实验结果为深入研究GRP94与肿瘤细胞发生、发展和转移的关系奠定了基础,在癌症的基因治疗上将具有一定意义。

To investigate the effect of specially down-regulated GRP94 on the biological properties of human colorectal cancer cell line CCL229 in vitro, a ribozyme targeted against the translation initiation of GRP94 mRNA were introduced into CCL229 cells by lipid-mediated DNA-transfection. After successfully setting up the stable cell line , we researched the change in the biological properties of this cell line. The results reveal that (1) The grow rate of transfected CCL229 significantly decreased after being induced by A23187 for 16h. (2) The aggregation capacity of induced ribozyme-expressing cells was lower than that of the control (P <0.01or P<0.05) . (3) Treatment of ribozyme-expressing cells with A23187 can induce a remarkable G0/ G1 arrest. Consequently, we can draw the conclusion that GRP94 is connected with the growth and invasion of colorectal cancer cells under stressed conditions; there was some alteration in the biological properties of human colorectal cancer cell line CCL229 when the expression of GRP94 was down-regulated specifically. Not only did our experiment deepen the understanding on the association of tumorgenesis, deterioration and metastasis with GRP94, it also showed light on gene therapy of cancer.