产纤维素酶菌种TP1202的选育及产酶条件研究

Selection of the cellulase producing strain TP1202 and the studyon the conditions for cellulase production

从腐木上分离到 1株纤维素酶活较高的野生纤维素酶产生菌TP0 1,经鉴定为绿色木霉 (Trichodermaviride)。以TP0 1为出发菌株 ,经紫外线、亚硝基胍、硫酸二乙酯和LiCl等物理化学诱变处理 ,最后得到 1株高产突变株TP12 0 2。通过对培养基中氮源、碳源、培养温度、培养时间、培养基的含水量、培养基的起始pH、培养基中葡萄糖含量的研究 ,测定TrichodermavirideTP12 0 2纤维素酶的CMC和滤纸酶活 ,找到了产纤维素酶的较佳条件 ,即 ,稻草粉 :麦麸 =4∶1,物料∶水份 =1∶0 75～ 1,以 (NH4 ) 2 SO4 或NH4 Cl为氮源 ,葡萄糖含量为 1%～ 2 % ,起始pH为 7 5 ,在 30℃下培养 96～ 12 0h左右 ,其酶活力为最高 ,每克干曲CMC酶和滤纸酶活分别达到 2 890 0U、6 0 4U ,是出发菌株的 3倍和 6倍。

A cellulase enzyme producing strain TP01 was isolated from a rotted wood It was identified as Trichoderma viride Based on the strain TP01,a mutated strain TP1202 is abtained through inducing treatment of ultraviolet light,NTG,DES and LiCl The factors of media including nitrogen sources,carbon sources,cultural temperatures,water content,original pH,cultural time and glucose content was studied for producing cellulase by Trichoderma viride TP1202 The results showed that the better condition are:the ratio of strain and wheat bran equals 4:1,the ratio of material and water equqls 1∶0 75～1,with (NH 4) 2SO 4 or NH 4Cl as nitrogen sources,glucose content is 1%-2%,the original pH is 7 5,the cultural time is 96～120 hours,the cultural temperature is 30℃ CMCase and FPA get lighest 28900 CMCsse u/g dried substrate and 604 FPA u/g dried subistrate Cellulase activity of TP1202 was about 3 times and 6 times that of the parent strain