摘要
采用水提醇沉法提取川牛膝多糖粗品GPC,经685弱碱阴离子交换柱层析和Bio-Gel P2凝胶柱层析进一步纯化,得到川牛膝多糖RCP(refined Cyathula officinalis Kuan polysaccharide).紫外扫描、高效液相色谱、聚丙烯酰胺盘状凝胶电泳证明RCP为均一组分.质谱图显示RCP的分子量(Mr)主要分布在1000～2200.薄层正交试验确定了RCP完全水解的最优条件.薄层层析及高效液相色谱-蒸发光散射检测(HPLC-ELSD)技术揭示,RCP单糖组成为D-果糖和D-葡萄糖.
Polysaccharide RCP was isolated and purified from Cyathula officinalis Kuan by hot water extraction, alcohol precipitation , 685 alkalescent anion exchange resin and Bio-Gel P2 polyacrylamide gel chromatography. Its homogeneity was proved by ultraviolet scanning, polyacrylamide disc electrophoresis and HPLC. Mass spectrum of RCP showed its molecular weight ranged mainly from 1 000 to 2 200. The optimal complete hydrolysis condition of RCP was established by TLC-orthogo-nal design. Its monosaccharide composition was determined by TLC and HPLC-ELSD, indicating RCP consisted of D-fructose and D-glucose. Fig 11, Tab 2, Ref 18
出处
《应用与环境生物学报》
CAS
CSCD
2003年第2期141-145,共5页
Chinese Journal of Applied and Environmental Biology
基金
中国科学院知识创新工程项目
中国科学院成都地奥科学基金(DASF)资助项目~~
关键词
川牛膝多糖
分离
纯化
单糖组成
药材
水提醇沉法
polysaccharide
Cyathula officinalis Kuan
isolation and purification
TLC-orthogonal design
monosaccharide composition
