青霉菌对活性艳蓝KN-R的吸附作用(英)

BIOSORPTION OF REACTIVE BRILLIANT BLUE KN-R BY PENICILLIUM X5

研究了青霉菌(Penicillium X5)对活性艳蓝KN-R的吸附作用.通过对培养液的波谱分析和宏观现象的观察,结果表明,在72 h内,脱色是由吸附引起的.当染料的浓度为100 mg/L时,活菌体对染料的吸附率可达88.66％.本实验还研究了对实际应用和吸附过程有影响的几个因素,包括葡萄糖、NaCl、温度和pH.结果表明:葡萄糖浓度在0～20g/L时,随着葡萄糖浓度的增加,菌体的干重相应增加,说明对活性艳蓝KN-R的吸附具有促进作用,但浓度在10～20g/L时,吸附作用不显著;而随着NaCl浓度(0～2％)的增加,吸附率却显著降低.最佳脱色温度为25℃,pH为4.0.活菌体与死菌体的生物吸附均符合Langmuir方程,活菌体比死菌体具有更好的吸附性能.吸附在菌丝体上的染料可以用甲醇进行洗脱,菌丝球在下次使用前用蒸馏水冲洗至pH中性,此菌丝球可重复使用3次.

The adsorption of Reactive Brilliant Blue KN-R by Penicillium X5 was studied. Through the spectra of excellular fluid and macroscopic observation, it was indicated that the decolorization was due to biosorption. When the concentration of Reactive Brilliant Blue KN-R was 100 mg/L, the adsorption rate of dye by the growing mycelium reached 88. 66%. Four factors were investigated including glucose and salt concentrations, temperature and pH, which would affect practical application and adsorption process. The results showed that with increasing of glucose concentration ranging between 0-20 g/L, the dry weight of mycelium pellets increased accordingly, implying glucose could accelerate biosorption of Reactive Brilliant Blue KN-R, and while the salinity increased within 0 ~ 2% , the adsorption rate decreased. The optimum pH and temperature of decolorization were 4. 0 and 25℃ , respectively. Biosorption process of growing mycelium and dead mycelium both corresponded with Langmuir model. The growing mycelium had better adsorptive capacity than dead mycelium. Before further adsorption tests, the dye was effectively eluted from biomass by methanol solution, and the biomass was regenerated by washing with deionized water till pH 7.0. The mycelia pellets could be used for three cycles of biosorption. Fig 8, Tab 2, Ref 11