转溶菌酶基因水稻稻瘟病抗谱分析

Analysis of Resistant Spectrum to Rice Blast in Transgenic Rice Lines Introduced Lysozyme Gene from T4 Phage

用来源于云南各地属于 4 8个稻瘟病菌 (Magnaporthegrisea)生理小种的 6 3个菌株 ,对受体品种南 2 9及其 10个T0 代转溶菌酶基因水稻植株繁衍的 36个T5代品系进行温室接种鉴定。结果表明 ,受体品种南 2 9对38.1%的菌株 (2 4个 )表现抗病 ,转基因品系对 72 %以上的菌株表现抗病 ,对稻瘟病的抗性比对照大幅度提高 ,证明溶菌酶基因对稻瘟病具有一定的广谱抗性 ;不同T0 代植株甚至同一植株繁衍的品系对稻瘟病的抗性并不相同。大田稻瘟病诱发鉴定结果证实 ,转基因水稻叶稻瘟和穗稻瘟抗性均比受体对照大幅度提高 ,但抗叶稻瘟的转基因品系不一定抗穗颈稻瘟 。

The receptor cultivar Nan 29 and thirty six rice lines derived from ten T 0 generation transgenic plants harboring lysozyme gene were challenged in greenhouse by inoculating 63 isolates belonging to 48 races of Magnaporthe grisea from Yunnan Province. The recptor cultivar Nan29 exhibited resistance to 38.1% (24 isolates) of isolates inoculated in this experiment, and the transgenic rice lines more than 72% of isolates. The resistance of the transgenic rice lines to rice blast enhanced compared with the receptor cultivar. The results indicated that the transgenic rice lines possessed wide spectrum resistance against various rice blast races and the resistant spectrum of rice lines were different although some lines derived from same T 0 plant. The transgenic rice lines exhibited also high resistance to leaf and neck blast in the disease field evaluation, but not all of resistant lines against leaf blast were resistant to neck blast.