小菜蛾羧酸酯酶基因的克隆及其序列分析

Cloning and Sequence Analysis of Carboxylesterase Gene in Diamondback Moth,Plutella xylostella (Lepidoptera:Plutellidae)

利用CODEHOP设计简并引物 ,通过反转录 多聚酶链式反应 (RT PCR)克隆小菜蛾Plutellaxylostella抗性种群中抗性相关的羧酸酯酶基因 ,随机挑取测序 5个阳性克隆进行测序 ,测序结果经过blastx比较 ,发现所获得的大约 42 0bp的基因片断均为羧酸酯酶基因 ,与双翅目昆虫蚊子的氨基酸序列同源性达 85

A pair of degenerate primers were designed by CODEHOP and were used to clone the carboxylesterase gene related with insecticide resistance in the resistant population of diamondback moth,Plutella xylostella (L.) using RT-PCR technique.Five positive clones were selected randomly and sequenced.After the blastx comparison,the partial cDNAs were confirmed that they were carboxylesterase gene and they have high identity (>85%) with Diptera insect mosquito.;