大豆异黄酮与叶酸对大鼠神经管畸形的保护作用

Protective effect of soybean isoflavones coordinated with folic acid on rats neural tube defects

目的 研究大豆异黄酮的抗氧化作用及其与叶酸配伍对大鼠神经管畸形的保护性作用。方法 以环磷酰胺制备大鼠神经管畸形的模型 ,生理盐水作对照组 ,另外设单独叶酸组、叶酸配伍大豆异黄酮共 5个干预组 (叶酸的量固定不变 ) ,分别进行抗氧化指标、胎鼠畸形发生率、生长发育指标和光镜及透射电镜的检测。结果 ( 1)干预组较模型组孕鼠血清SOD含量增加、MDA含量降低 ,NO含量有所下降 ,且与大豆异黄酮有剂量 -反应关系 ;( 2 )胎鼠大体形态结果显示 ,干预实验组胎鼠脑膨出发生率较模型组均有下降 ,并以 40mg/ (kg·bw)的大豆异黄酮配伍叶酸组下降最明显 ;( 3)干预组胎鼠的各项生长发育指标均较模型组有所增长或增重 ;( 4)光镜病理切片显示 ,干预组细胞碎片和小群凋亡细胞聚集的现象较模型组明显减少 ;( 5 )透射电镜结果可以看见细胞凋亡的严重程度以及凋亡细胞的数量较模型组有明显改善。结论 大豆异黄酮在一定剂量时与叶酸配伍可以较单独补充叶酸更为有效地预防大鼠神经管畸形的发生。

Objective To observe the protective effects of soybean isoflavones coordinated with folic acid and mechanism on rats.Methods The pregnant rats were randomly divided into positive and negative control groups and five expreimental groups according to their body weight,cyclophosphamide were given to made the neural tube defects model.The biochemical indices were checked,the morphological changes of the embryos were checked through optical microscopy and transmission electron microscopy.Results (1)The SOD activities increased while MDA?NO declined in every prevention groups.The changes showed dose-effect response relationship with soybean isoflavones;(2)The frequency of abnormalty decreased,moreover there was the best effect in the group of folic acid coordinated with 40?mg/L soybean isoflavones;(3)The develop mental index of embories significantly improved compared with the model-making grouop;(4)Under optical microcopy and transmission electron microscopy,the nucleus and the organelles were not significantly damaged or the impairment were diminished.Conclusion Soybean isoflavones showed strong antioxidant effect coordinated with folic acid in a conformated-dose which can decreased frequency of abnormalty more effectively than folic acid alone.